Evaluation of genetic diversity in different genotypes of Gerbera jamesonii Bolus using random amplified polymorphic DNA (RAPD) markers
نویسندگان
چکیده
منابع مشابه
Study of Genetic Diversity in Wheat (triticum Aestivum L.) Using Random Amplified Polymorphic Dna (rapd) Markers
Twelve wheat genotypes developed through hybridization programme were screened for genetic diversity through RAPD marker. A total of 102 loci were amplified with 14 primers out of which 91 (89.2%) were polymorphic and only 11(10.8%) were monomorphic. Fragments size ranged from 142bp-5.3kb and fragments produced by various primers ranged from 1-11 with an average of 7.1 fragments per primer. The...
متن کاملRandom Amplified Polymorphic DNA (RAPD) Markers
Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
متن کاملEvaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line
An attempt was made to evaluate the utility of a method which employs semi-specific PCR using partially specific primers for the coding sequence (ET) at the exon-intron contact and of the RAPD method to identify eight Polish cultivars of gerbera. It was demonstrated that the PCR method which employs semi-specific primers is as simple and economical as the RAPD method, simultaneously the images ...
متن کاملStudy of Genetic Diversity among Philodendron Varieties by Randomly Amplified Polymorphic DNA Markers
In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymo...
متن کاملRandom amplified polymorphic DNA (RAPD) markers and its applications
In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD is a modification of the PCR in which a single, short and arbitrary oligonucleotide primer, able to anneal and prime at multiple locations throughout the genome, can produce a spectrum of a...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2014
ISSN: 1684-5315
DOI: 10.5897/ajb2013.13347